Generalised mathematical model for interpretation of frap experiments

Abstract

A novel mathematical approach for quantitative investigation of protein dy- namics in living cells with °uorescence recovery after photobleaching (FRAP) experiments has been developed. Existing FRAP Їtting models are based on the speciЇc chemical reaction equations and often exhausting for parameters Їtting. Our model allows to study di®erent biomolecular processes and can be used as a non-Їtting procedure for quantitative FRAP analysis. The mathematical for- malism is based on the theory of stochastic processes. The derived model was validated on the examples of binding and actin polymerization reactions.